simultaneous determination of paracetamol, 4-aminophenol, 4-chloroacetanilid, benzyl alcohol,benzaldehyde and edta by hplc methodin paracetamol injection ampoule

paracetamol that is known as acetaminophen have the most consume as an analgesic and antipyreticdrug in the world. that is formulated in single compound or mixture at many forms such astablets, syrups, suspensions and drops. the last form is intravenous injections. paracetamol derivedfrom 4-minophenol which is synthesized by acylated the p-acetaminophenol and acetic anhydride.4-aminophenol is the main impurity at manufacturing of paracetamol which could produce byhydrolysis during storage or synthesis under normal conditions (temperature, ph, etc.). also,4-chloroacetanilid may be observed as an impurity in the raw material of paracetamol synthesis.benzyl alcohol is a preservative that used in paracetamol for injection. it will be very important ifthere are analytical techniques to measuring paracetamol and its degradation products accurately andeasily. undoubtedly the most important and widely used, separation technique is chromatography.there are several reports about separation and quantitative determination of paracetamol lonely orsimultaneous determination of paracetamol and 4-aminophenol. in this paper investigated simultaneousdetermination of paracetamol, 4-aminophenol, 4-chloroacetanilid, benzyl alcohol, benzaldehyde,and edta in paracetamol for injection ampoules by high performance liquid chromatography. bychanging the ratio of mixing methanol and acetonitrile as mobile phase at the wavelength of 215 nmand ph=3 separation of all compounds were completely done.